Analysis Note

ControlDifferentiated ReNcell VMs

Application

Research CategoryNeuroscience

Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected GFAP in adult mouse brain tissue.

Research Sub CategoryDevelopmental Neuroscience

Anti-GFAP Antibody, Cy3 Conjugate is an antibody against GFAP for use in IC, IH.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Glial fibrillary acidic protein is a class-III intermediate filament. GFAP is the main constituent of intermediate filaments in astrocytes and serves as a cell specific marker that distinguishes differentiated astrocytes from other glial cells during the development of the central nervous system.

Immunogen

Purified glial filament protein (Debus, E., 1983).

Physical form

Protein A purified

Purified mouse monoclonal IgG1 conjugated to Cy3 in PBS with 0.1% sodium azide and 15 mg/mL BSA.

Quality

Evaluated by Immunocytochemistry in differentiated ReNcell VMs.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected GFAP in differentiated ReNcell VMs.

Specificity

The antibody reacts with mouse GFAP. In tissue sections this
antibody stains astrocytes and Bergman glia cells (Debus, E., 1983).

Storage and Stability

Maintain refrigerated at 2-8 °C protected from light in undiluted aliquots for up to 6 months from date of receipt.

Target description

50 kDa Observed (Using unconjugated antibody MAB3402)